ZETA: Utilization of Novel kLa Measurement Procedure for Bioreactor Characterization & Optimization
Today, in line with the evolution of bio-processing techniques to satisfy the concepts on Pharma 4.0, there is heavy emphasis on cell line and expression system improvements. With this background, the demands on bioreactors have become more exacting over recent years, requiring paradigm shifts in manufacturing efficiencies. Continuous optimization, design improvements and characterization of bioreactors are crucial factors placing high demands for innovation in bio-pharmaceutical manufacturing. Therefore, it is inevitable that a focus is placed on new approaches in plant engineering to keep pace with the technological developments of the bio-pharmaceutical industry.
The mass transfer coefficient (kL) and the value of the inter facial area (a), is used to characterize bioreactor systems for their oxygen transfer capability. The kLa-value and the mixing time are among the most important performance indicators for bioreactors. The calculation and modelling of the kLa-value and mixing time is extremely complex in highly aerated systems.
This paper describes a new methodology for measuring the kLa value, to accurately determine the coefficient. The valuable insights obtained allow for improved bioreactor characterization, thus making it easier to meet regulatory requirements. Furthermore, this paper cites three case studies outlining the benefits in the practical application of the new methodology and was written by Thomas Maischberger, PhD at ZETA.